In the body, dangerous naturally occurring substances called free radicals pose a risk of harm to many tissues. The body deploys an “antioxidant defense system” to hold them in check. Glutathione, a protein made from the amino acids cysteine, glutamic acid, and glycine, is one of the most important elements of this defense system.
Glutathione does much of its work in the liver, although it is also found elsewhere in the body. Besides fighting free radicals, it helps keep various essential biological molecules in a chemical state called “reduced” (as opposed to “oxidized”). In addition, glutathione can act on toxins such as pesticides, lead, and dry cleaning solvents, transforming them in such a way that the body can excrete them more easily.
Nutrients such as
also help neutralize free radicals. In the 1990s, such
were widely promoted for preventing a variety of diseases, including cancer and heart disease. Unfortunately, this hope has largely floundered as the results of large, reliable studies have come in. During this period, oral glutathione became popular as an additional antioxidant supplement but glutathione is not absorbed when taken by mouth, so such supplements are almost certainly useless. It may be possible, however, to raise glutathione levels in the body by taking other supplements, such as
. Whether doing so would offer any health benefits remains unclear.
There is no dietary requirement for glutathione. The body makes it from scratch, utilizing vitamins and common amino acids found in food.
Glutathione levels in the body are reduced by cigarette smoking. Various diseases are also associated with reduced levels of glutathione, including cancer,
A typical recommended dose of oral glutathione is ranges from 50 mg (and higher) per day. As a general rule, glutathione taken by mouth is destroyed.
Therefore, no matter what the dose, it won’t make any difference.
It is possible that some glutathione may be absorbed if it is held in the mouth and allowed to dissolve, but this has not been well studied.
A more promising method for raising glutathione levels in the body involves taking supplemental cysteine or antioxidant supplements. Evidence suggests that cysteine (often supplied in the form of whey protein, which is high in cysteine) can raise glutathione levels in people with
In addition, because
has overlapping functions with glutathione, vitamin C supplements may spare some of the body’s glutathione from being used up, thereby increasing its levels in the body.
The antioxidant supplement
appears to raise glutathione levels as well.
Other supplements that might raise glutathione levels include
Various websites promote glutathione for a wide variety of health problems, from preventing aging to enhancing sports performance. However, oral glutathione supplements may be useless for any condition since they do not appear to be absorbed or stay in the body for any length of time.
There is a bit of evidence that injected glutathione might offer a few heath benefits, such as preventing blood clots during surgery,
reducing the side effects and increasing the effectiveness of
drugs such as cisplatin,
and alleviating symptoms of early
Although oral glutathione is not likely to provide the same benefits, it is at least theoretically possible that taking the nutrients described in the previous section (and thereby raising glutathione levels indirectly) could offer similar benefits. However, there is no direct evidence to indicate that this hypothesis is true.
A small randomized trial showed some promise that glutathione may remain in the body with regular use. Fifty-four adults were given a glutathione supplement (250 mg per day or 1000 mg per day) or placebo for 6 months. Glutathione levels in blood increased in those taking the supplement, with a greater response seen in those taking the higher dose. The supplement group also demonstrated a reduction in oxidative stress. However, blood levels of glutathione returned to their original baseline level within 1 month of stopping the supplement.34
Firm glutathione dosages have not been established, though most supplements range up to 1,000 mg per day. There are no known side effects or toxicities, but some people may be more sensitive to it than others. Talk to a healthcare professional before taking any glutathione supplements.
White AC, Thannickal VJ, Fanburg BL. Glutathione deficiency in human disease.
J Nutr Biochem
Witschi A, Reddy S, Stofer B, Lauterburg BH. The systemic availability of oral glutathione.
Eur J Clin Pharmacol
Hunjan MK, Evered DF. Absorption of glutathione from the gastro-intestinal tract.
Biochim Biophys Acta
Bounous G. Whey protein concentrate (WPC) and glutathione modulation in cancer treatment.
Bounous G, Gervais F, Amer V, Batist G, Gold P. The influence of dietary whey protein on tissue glutathione and the diseases of aging.
Clin Invest Med
Micke P, Beeh KM, Schlaak JF, Buhl R. Oral supplementation with whey proteins increases plasma glutathione levels of HIV-infected patients.
Eur J Clin Invest
Watanabe A, Okada K, Shimizu Y, et al.
Nutritional therapy of chronic hepatitis by whey protein (non-heated).
Henning SM, Zhang JZ, McKee RW, Swendseid ME, Jacob RA. Glutathione blood levels and other oxidant defense indices in men fed diets low in vitamin C.
Johnston CS, Meyer CG, Srilakshmi JC. Vitamin C elevates red blood cell glutathione in healthy adults.
Am J Clin Nutr
Arivazhagan P, Ramanathan K, Panneerselvam C. Effect of DL-alpha-lipoic acid on glutathione metabolic enzymes in aged rats.
Bunin AIa, Filina AA, Erchev VP. A glutathione deficiency in open-angle glaucoma and the approaches to its correction.
Han D, Handelman G, Marcocci L, et al.
Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization.
Hultberg B, Andersson A, Isaksson A. Lipoic acid increases glutathione production and enhances the effect of mercury in human cell lines.
Packer L, Kraemer K, Rimbach G.
Molecular aspects of lipoic acid in the prevention of diabetes complications.
Sen CK, Roy S, Han D, Packer L.
Regulation of cellular thiols in human lymphocytes by alpha-lipoic acid: a flow cytometric analysis.
Free Radic Biol Med
Droge W, Breitkreutz R. Glutathione and immune function.
Proc Nutr Soc
De Rosa SC, Zaretsky MD, Dubs JG, et al.
N-acetylcysteine replenishes glutathione in HIV infection.
Eur J Clin Invest
Witschi A, Junker E, Schranz C, Speck RF, Lauterburg BH. Supplementation of N-acetylcysteine fails to increase glutathione in lymphocytes and plasma of patients with AIDS.
AIDS Res Hum Retroviruses
Droge W, Breitkreutz R. N-acetyl-cysteine in the therapy of HIV-positive patients.
Curr Opin Clin Nutr MetabCare
Amores-Sanchez MI, Medina MA. Glutamine, as a precursor of glutathione, and oxidative stress.
Wang ST, Chen HW, Sheen LY, Lii CK. Methionine and cysteine affect glutathione level, glutathione-related enzyme activities and the expression of glutathione S-transferase isozymes in rat hepatocytes.
Vendemiale G, Altomare E, Trizio T, et al. Effects of oral S-adenosyl-L-methionine on hepatic glutathione in patients with liver disease.
Scand J Gastroenterol.
Molloy J, Martin JF, Baskerville PA, et al. S-nitrosoglutathione reduces the rate of embolization in humans.
Campbell KC, Larsen DL, Meech RP, Rybak LP, Hughes LF.
Glutathione ester but not glutathione protects against cisplatin-induced ototoxicity in a rat model.
J Am Acad Audiol
Smyth JF, Bowman A, Perren T, et al. Glutathione reduces the toxicity and improves quality of life of women diagnosed with ovarian cancer treated with cisplatin: results of a double-blind, randomised trial.
Cascinu S, Cordella L, Del Ferro E, et al. Neuroprotective effect of reduced glutathione on cisplatin-based chemotherapy in advanced gastric cancer: a randomized double-blind placebo-controlled trial.
J Clin Oncol
Lenzi A, Picardo M, Gandini L, et al. Glutathione treatment of dyspermia: effect on the lipoperoxidation process.
Lenzi A, Culasso F, Gandini L, et al. Placebo-controlled, double-blind, cross-over trial of glutathione therapy in male infertility.
Lenzi A, Gandini L, Picardo M, Tramer F, Sandri G, Panfili E.
Lipoperoxidation damage of spermatozoa polyunsaturated fatty acids (PUFA): scavenger mechanisms and possible scavenger therapies.
. 2000 Jan 1;5:E1-E15.
Lenzi A, Gandini L, Picardo M. A rationale for glutathione therapy.
Lenzi A, Picardo M, Gandini L, Dondero F.
Lipids of the sperm plasma membrane: from polyunsaturated fatty acids considered as markers of sperm function to possible scavenger therapy.
Bharath S, Hsu M, Kaur D, Rajagopalan S, Andersen JK. Glutathione, iron and Parkinson's disease.
Sechi G, Deledda MG, Bua G, et al. Reduced intravenous glutathione in the treatment of early Parkinson’s disease.
Prog Neuropsychopharmacol Biol Psychiatry
Richie JP Jr, Nichenametla S, et al. Randomized controlled trial of oral glutathione supplementation on body stores of glutathione. Eur J Nutr. 2014 May 5 [Epub ahead of print].
Last reviewed September 2014 by EBSCO CAM Review Board
Please be aware that this information is provided to supplement the care provided by your physician. It is neither intended nor implied to be a substitute for professional medical advice. CALL YOUR HEALTHCARE PROVIDER IMMEDIATELY IF YOU THINK YOU MAY HAVE A MEDICAL EMERGENCY. Always seek the advice of your physician or other qualified health provider prior to starting any new treatment or with any questions you may have regarding a medical condition.
Copyright © EBSCO Publishing. All rights reserved.